Characterization of Cold-Tolerant Saccharomyces cerevisiae Cheongdo Using Phenotype Microarray

The cold-tolerant yeast is industrially useful for lager fermentation, high-quality wine, and frozen dough production. Cheongdo is a recent isolate from frozen peach samples which has a good fermentation performance at low temperatures and desirable flavor profiles. Here, phenotype microarray was used to investigate industrial potentials of Cheongdo using 192 carbon sources. Compared to commercial wine yeast EC1118, Cheongdo showed significantly different growth rates on 34 substrates. The principal component analysis of the results highlighted that the better growth of Cheongdo on galactose than on EC1118 was the most significant difference between the two strains. The intact gene and the galactose fermentation performance at a low temperatures suggested that Cheongdo is a promising host for industrial fermentation rich in galactose, such as lactose and agarose.