Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

•NRP is a rapid, cost-effective and simple method for reductive capacity assessment.•NRP is compatible with high-throughput screening of solid and liquid biological samples.•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis. We propose a rapid, simple,...

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Veröffentlicht in:MethodsX 2022-01, Vol.9, p.101611-101611, Article 101611
Hauptverfasser: Homolak, Jan, Kodvanj, Ivan, Babic Perhoc, Ana, Virag, Davor, Knezovic, Ana, Osmanovic Barilar, Jelena, Riederer, Peter, Salkovic-Petrisic, Melita
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Sprache:eng
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Zusammenfassung:•NRP is a rapid, cost-effective and simple method for reductive capacity assessment.•NRP is compatible with high-throughput screening of solid and liquid biological samples.•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis. We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.•NRP is a rapid, cost-effective, and simple method for reductive capacity assessment•NRP is compatible with a high-throughput screening of solid and liquid biological samples•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis [Display omitted]
ISSN:2215-0161
2215-0161
DOI:10.1016/j.mex.2021.101611