Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment
•NRP is a rapid, cost-effective and simple method for reductive capacity assessment.•NRP is compatible with high-throughput screening of solid and liquid biological samples.•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis. We propose a rapid, simple,...
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Veröffentlicht in: | MethodsX 2022-01, Vol.9, p.101611-101611, Article 101611 |
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Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | •NRP is a rapid, cost-effective and simple method for reductive capacity assessment.•NRP is compatible with high-throughput screening of solid and liquid biological samples.•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis.
We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.•NRP is a rapid, cost-effective, and simple method for reductive capacity assessment•NRP is compatible with a high-throughput screening of solid and liquid biological samples•HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis
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ISSN: | 2215-0161 2215-0161 |
DOI: | 10.1016/j.mex.2021.101611 |