Characterization of Mungbean CONSTANS-LIKE Genes and Functional Analysis of CONSTANS-LIKE 2 in the Regulation of Flowering Time in Arabidopsis

( ) genes play important roles in the regulation of plant growth and development, and they have been analyzed in many plant species. However, few studies have examined genes in mungbean ( ). In this study, we identified and characterized 31 mungbean genes whose proteins contained B-Box domains. Fourteen were designated as genes and were distributed on 7 of the 11 mungbean chromosomes. Based on their phylogenetic relationships, were clustered into three groups (I, II, and III), which contained 4, 6, and 4 members, respectively. The gene structures and conserved motifs of the genes were analyzed, and two duplicated gene pairs, and , were identified. A total of 82 -acting elements were found in the promoter regions, and the numbers and types of -acting elements in each promoter region differed. As a result, the expression patterns of varied in different tissues and throughout the day under long-day and short-day conditions. Among these genes, showed a close phylogenetic relationship with and displayed daily oscillations in expression under short-day conditions but not long-day conditions. In addition, overexpression of accelerated flowering in under short-day conditions by affecting the expression of the flowering time genes and . Our study lays the foundation for further investigation of gene functions.