INCIDENCE AND CLINICO-PATHOLOGICAL STUDIES ON CANINE BABESIOSIS IN AND AROUND AKOLA, MAHARASHTRA, INDIA

Canine babesiosis is a tick-borne hemoprotozoan disease of canines. Two main infective agents of the disease are intra-erythrocytic i.e. Babesia vogeli and Babesia gibsoni. In the present study, a total of 145 numbers of dogs (90 male and 55 female) in and around Akola, Maharashtra were screened from January 2022 to September 2022 for Babesiosis and 18 animals (12.41%) were found clinically and cytologically positive. The incidence of babesiosis was higher in females (11/145, 7.58%) than in males (07/ 145, 4.83%). Out of 18 positive samples, 14 (9.65%) and 4 (2.75%) animals were found infected with small and large forms of Babesia spp., respectively. Further small form was confirmed as B. gibsoni and the large form was confirmed as B. vogeli by employing PCR assay. All the cases with babesiosis revealed moderate to severe regenerative anemia with occasional basophilic stippling and Howell jolly bodies with hemoglobin ranging from 7 to 11 g/dL. Characteristic clinical features were observed among 18 positive clinical cases. The present investigation reports the incidence of B. vogeli and B. gibsoni in canines in and around Akola of Maharashtra state of India.