Hsa-miR-217 Inhibits the Proliferation, Migration and Invasion in Non-small-cell Lung Cancer Cells Via Targeting SIRT1 and p53/KAI1 Signaling

Brain metastasis is a major cause for cancer death in patients with lung cancer. Sirtuin 1 (SIRT1) and hsa-miR-217 has been identified to mediate the development of non-small cell lung cancer (NSCLC). We performed this study to investigate the roles of hsa-miR-217, its target SIRT1, as well as P53/KAI1 axis in the brain metastasis from NSCLC. This is a cell culture study. Human pulmonary adenocarcinoma brain metastasis cell line PC-14/B were incubated and treated with constructed lentiviral plasmids expressing miR-217 and/or SIRT1. BEAS-2B cell line was used as a control. The targeted regulation of miR-217 to SIRT1 was examined using dual luciferase reporter assay. Cell proliferation, migration, invasion and the expression of relate proteins were detected to examine the effect of miR-217/SIRT1 expression on metastasis. PC-14/B cells expressed higher SIRT1 and lower p53 and KAI1 compared with BEAS-2B control cells (P