Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus

The incidence of dengue in Latin America has increased dramatically during the last decade. Understanding the pathogenic mechanisms in dengue is crucial for the identification of biomarkers for the triage of patients. We aimed to characterize the profile of cytokines (IFN-γ, TNF-α, IL-1β, IL-6, IL-1...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Viruses 2021-09, Vol.13 (9), p.1789
Hauptverfasser: Fiestas Solórzano, Victor Edgar, da Costa Faria, Nieli Rodrigues, Dos Santos, Caroline Fernandes, Corrêa, Gladys, Cipitelli, Márcio da Costa, Dornelas Ribeiro, Marcos, de Souza, Luiz José, Damasco, Paulo Vieira, da Cunha, Rivaldo Venâncio, Dos Santos, Flavia Barreto, de Oliveira Pinto, Luzia Maria, de Azeredo, Elzinandes Leal
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The incidence of dengue in Latin America has increased dramatically during the last decade. Understanding the pathogenic mechanisms in dengue is crucial for the identification of biomarkers for the triage of patients. We aimed to characterize the profile of cytokines (IFN-γ, TNF-α, IL-1β, IL-6, IL-18 and IL-10), chemokines (CXCL8/IL-8, CCL2/MCP-1 and CXCL10/IP-10) and coagulation mediators (Fibrinogen, D-dimer, Tissue factor-TF, Tissue factor pathway inhibitor-TFPI and Thrombomodulin) during the dengue-4 epidemic in Brazil. Laboratory-confirmed dengue cases had higher levels of TNF-α ( < 0.001), IL-6 ( = 0.005), IL-10 ( < 0.001), IL-18 ( = 0.001), CXCL8/IL-8 ( < 0.001), CCL2/MCP-1 ( < 0.001), CXCL10/IP-10 ( = 0.001), fibrinogen ( = 0.037), D-dimer ( = 0.01) and TFPI ( = 0.042) and lower levels of TF ( = 0.042) compared to healthy controls. A principal component analysis (PCA) distinguished between two profiles of mediators of inflammation and coagulation: protective (TNF-α, IL-1β and CXCL8/IL-8) and pathological (IL-6, TF and TFPI). Lastly, multivariate logistic regression analysis identified high aspartate aminotransferase-to-platelet ratio index (APRI) as independent risk factors associated with severity (adjusted OR: 1.33; 95% CI 1.03-1.71; = 0.027), the area under the receiver operating characteristics curve (AUC) was 0.775 (95% CI 0.681-0.869) and an optimal cutoff value was 1.4 (sensitivity: 76%; specificity: 79%), so it could be a useful marker for the triage of patients attending primary care centers.
ISSN:1999-4915
1999-4915
DOI:10.3390/v13091789