In vitro nuclear maturation of bovine oocytes selected by the brilliant cresyl blue method

In vitro nuclear maturation of bovine oocytes selected by the brilliant cresyl blue method

The objective was to investigate in vitro nuclear maturation of bovine oocytes selected by Brilliant Cresyl Blue (BCB) after maintenance in Talp-Hepes medium. Cumulus oocyte complexes (COCs) obtained from slaughterhouse ovaries were randomly assigned to 6 treatments. I. As a percentage of oocytes th...

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Veröffentlicht in:Revista colombiana de ciencia animal recia 2017-12, Vol.9 (2), p.345-354
Hauptverfasser: Rafael Otero A, Eduardo Da Costa P, Emílio Pereira M
Format: Artikel
Sprache:eng
Schlagworte:
Azul cresil brilhante
Maturação in vitro
Ovócitos
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Zusammenfassung:The objective was to investigate in vitro nuclear maturation of bovine oocytes selected by Brilliant Cresyl Blue (BCB) after maintenance in Talp-Hepes medium. Cumulus oocyte complexes (COCs) obtained from slaughterhouse ovaries were randomly assigned to 6 treatments. I. As a percentage of oocytes that presented nuclear configuration of MI (Metaphysis I) at the end of IVM, T3 and T5 were higher than T1, T2 and T4 (p
ISSN:2027-4297
DOI:10.24188/recia.v9.n2.2017.617