miR-142-3p inhibits cerulein-induced apoptosis of rat pancreatic exocrine cell line AR42J by regulating Hmgb1

Objective To investigate the effect of miR-142-3p on the apoptosis of rat pancreatic exocrine cell line AR42J by regulating Hmgb1. Methods AR42J cells were divided into blank group (blank), acute pancreatitis model group (AP, 100 nmol/L cerulein for 24 h), and then transfected with miR-142-3p mimics, mimics NC,miR-142-3p inhibitor and inhibitor NC, respectively. The cells in the model group were recorded as miR-142-3p mimics group, mimics NC group, miR-142-3p inhibitor group and inhibitor NC.The expression of miR-142-3p in cells was detected by RT-qPCR. The protein expressions of HMGB1, caspase-3, Bax and Bcl-2 were detected by Western blot. Hoechst staining was used to determine cell apoptosis. The apoptosis rate of cells was detected by flow cytometry. The targeting relationship between miR-142-3p and Hmgb1 was determined by dual luciferase reporter gene assay. Results Compared with blank control group, the expression level of miR-142-3p in the AP group was significantly down-regulated (P＜ 0.01), the expres