Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Keywords: Human antibodies, SHM, CRISPR, CRISPR-X, AID, Tetramers, HLA, Cytofluorimetry

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Bibliographische Detailangaben
Veröffentlicht in:BMC biotechnology 2019-02, Vol.19 (1), p.14-14, Article 14
Hauptverfasser: Devilder, Marie-Claire, Moyon, Melinda, Gautreau-Rolland, Laetitia, Navet, Benjamin, Perroteau, Jeanne, Delbos, Florent, Gesnel, Marie-Claude, Breathnach, Richard, Saulquin, Xavier
Format: Artikel
Sprache:eng
Schlagworte:
AID
Anopheles
Antibodies
Antibodies - genetics
Antibodies - immunology
Antibody Affinity
Antigens
B cells
B-Lymphocytes - immunology
Biological products
Biopharmaceuticals
Cancer
CRISPR
CRISPR-Cas Systems
CRISPR-X
Cytidine Deaminase - genetics
Cytidine Deaminase - immunology
Directed Molecular Evolution
Evolutionary biology
Gene Editing
Genes
Genetic engineering
Genetic vectors
Genetics
HEK293 Cells
HLA Antigens - immunology
Human antibodies
Humans
Immune response
Life Sciences
Methodology
SHM
Somatic Hypermutation, Immunoglobulin
Tetramers
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Beschreibung
Zusammenfassung:Keywords: Human antibodies, SHM, CRISPR, CRISPR-X, AID, Tetramers, HLA, Cytofluorimetry
ISSN:1472-6750
1472-6750
DOI:10.1186/s12896-019-0504-z