Workflow in transgenic mice for the study of specific cancers associated with the post-menopausal state

Beta-hCG is associated with poor patient prognosis in several cancers, but the particular pathophysiology of beta-hCG in post-menopausal women remains unaddressed. Specific steps to culture Lewis lung carcinoma (LLC1) tumor cells are enumerated. Ovariectomy of syngeneic, beta-hCG transgenic mice is discussed, employing a protocol designed to ensure high survival. Implantation of LLC1 tumor cells in these mice is also described. The workflow can be easily adapted in the study of other cancers associated with the post-menopausal stratum. For complete details on the use and execution of this protocol, please refer to Sarkar et al. (2022).1 [Display omitted] •Lewis lung carcinoma cell cultures for assessing steroid toxicity•Optimal procedures for ovariectomizing beta-hCG transgenic mice•Implantation of Lewis lung carcinoma cells in transgenic mice•Use of ovariectomized beta-hCG transgenic mice to study post-menopausal cancers Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Beta-hCG is associated with poor patient prognosis in several cancers, but the particular pathophysiology of beta-hCG in post-menopausal women remains unaddressed. Specific steps to culture Lewis lung carcinoma (LLC1) tumor cells are enumerated. Ovariectomy of syngeneic, beta-hCG transgenic mice is discussed, employing a protocol designed to ensure high survival. Implantation of LLC1 tumor cells in these mice is also described. The workflow can be easily adapted in the study of other cancers associated with the post-menopausal stratum.