Development of a multilayer biodegradable active packaging based on nano emulsions, for the bio preservation of food

Multi-layer biodegradable active packaging is a new class of innovative food packaging containing bioactive compounds that are able to maintain food quality and extend shelf life preservation by releasing an active agent during storage. In this context, this study aims to develop and characterize three-layer biodegradable active films: 2 outer layers of ethylcellulose (EC) and a layer internal pectin containing nanoemulsions (NE) of trans-cinnamaldehyde (TC) as an antimicrobial agent. Trans-cinnamaldehyde (NE) nanoemulsions were prepared with soy lecithin as an agent. emulsifier to stabilize the TC and protect it for the duration of the film manufacturing. The average size and the zeta potential of the nanoemulsion droplets were 103.93 nm and -46 mV, respectively. The opacity of samples of monolayer and multilayer films decreased by 15.19 (A*mm-1) for the monolayer film of pectin incorporated by nanocapsules at 2.02 for multilayer films. In addition, the multilayer technique has made it possible to improve the mechanical properties of the films compared to monolayers with greater resistance breakage and greater extensibility. Inhibition tests on four bacteria representatives of bacteria pathogens and spoilage found in food industries have confirmed the antimicrobial activity, both, mono and multilayers. The work presented in this study offers perspectives for the fabrication packaging based on antimicrobial and biodegradable multilayer films. L’emballage actif biodégradable multicouche est une nouvelle classe d’emballages alimentaires innovants contenant des composés bioactifs qui sont capables de maintenir la qualité des aliments et de prolonger leur durée de conservation en libérant un agent actif pendant le stockage. Dans ce contexte, cette étude vise à développer et à caractériser des films actifs biodégradables tricouches : 2 couches extérieures d’éthylcellulose (EC) et une couche interne de pectine contenant des nanoémulsions (NE) de trans-cinnamaldéhyde (TC) comme agent antimicrobien. Les nanoémulsions de trans-cinnamaldéhyde (NE) ont été préparées avec la lécithine de soja en tant qu’agent émulsifiant afin de stabiliser le TC et le protéger pendant la durée de la fabrication du film. La taille moyenne et le potentiel zêta des gouttelettes de la nanoémulsion étaient respectivement de 103,93 nm et -46 mV. L’opacité des échantillons de films monocouches et multicouches a diminué de 15,19 (A*mm -1 ) pour le film monocouche de pectine incorporé par des