Molecular identification of Theileria species in cattle in Mosul city

This study showed that total percentage of infection with Theileria species in 100 blood samples of cattle in Mosul City was 38% with no significant differences between male and female of cattle. Cattle older than 1 year old had a high percentage of Thieleria infection 40% compared to younger cattle 34.28%, with no significant variations in Thieleria infection and age groups of cattle. Theileria measurement in red blood cells ranged from 0.5 to 2 microns, while parasitemia levels ranged from 0.2 to 15%, with an average of 6.572%. The results of molecular diagnosis confirmed the detection Theileria genus, Theileria annulata, and Theileria parva in blood samples of examined cattle and PCR revealed that the amplification products were 237bp, 690bp, and 700bp, respectively. In this study, we recorded new strains of Theileria in cattle of the Nineveh governorate by using the BLAST program consisting of three strains of T. annulata, one strain of Theileria ovis and two strains of Theileria parva which differs from the normal isolates in many nucleotides in different worlds. The phylogenetic tree showed the relative relation among the Theileria spp, the results matching species between 8 and 11, 1 and 4, 6 and 10, 3 and 9, and 2 and 12; the species 7 were more closely linked to 11 and 8, the species five similar to 6 and 10.