Genetic Variation in an Experimental Goldfish Derived From Hybridization

Owning to the extreme difficulty in identifying the primary generation (G ), the common ancestor of various twin-tail goldfish strains remains unclear. However, several authors have hypothesized that this ancestor may have been the crucian carp ( ). Previously, we generated an experimental hybrid go...

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Veröffentlicht in:Frontiers in genetics 2020-12, Vol.11, p.595959-595959
Hauptverfasser: Wang, Jing, He, Weiguo, Zeng, Jinfeng, Li, Lixin, Zhang, Guigui, Li, Tangluo, Xiang, Caixia, Chai, Mingli, Liu, Shaojun
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Sprache:eng
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Zusammenfassung:Owning to the extreme difficulty in identifying the primary generation (G ), the common ancestor of various twin-tail goldfish strains remains unclear. However, several authors have hypothesized that this ancestor may have been the crucian carp ( ). Previously, we generated an experimental hybrid goldfish (EG) from the interspecific hybridization of red crucian carp ( ♀, RCC) × common carp ( ♂, CC). Unlike either parent, EG possessed twin caudal fins similar to those of natural goldfish ( , NG). The genetic characteristics of EG, as well as the mechanisms underlying its formation, are largely unknown. Here, we identified the genetic variation in the gene that was associated with the formation of the twin-tail phenotype in EG: a stop codon mutation at the 127 amino acid. Furthermore, simple sequence repeat (SSR) genotyping indicated that, among the six alleles, all of the EG alleles were also present in female parent (RCC), but alleles specific to the male parent (CC) were completely lost. At some loci, EG and NG alleles differed, showing that these morphologically similar goldfish were genetically dissimilar. Collectively, our results demonstrated that genetic variations and differentiation contributed to the changes of morphological characteristics in hybrid offspring. This analysis of genetic variation in EG sheds new light on the common ancestor of NG, as well as on the role of hybridization and artificial breeding in NG speciation.
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2020.595959