Effects of Aloin on Proliferation, Apoptosis and Invasion of Esophageal Cancer KESY70 Cell Line

Objective To explore the effect of aloin on the proliferation, apoptosis and invasion of esophageal cancer KESY70 cell line. Methods Different concentrations of aloin were added into KESY70 cells which were randomly divided into five groups: untreated group (KESY70), control group (DMSO) and Aloin groups (10, 40, 80 μmol/L). CCK-8 assay was used to test the viability and proliferation of KESY70 cells. The apoptosis was detected by flow cytometry. Transwell assay was performed to analyze the invasion of KESY70 cells. The expression levels of PCNA, cleaved caspase-3 and MMP-9 were checked by Western blot. Results Cell viabilities of 40, 80 and 120 μmol/L Aloin groups were remarkably decreased, compared with DMSO group(P