Complex sputum microbial composition in patients with pulmonary tuberculosis

An increasing number of studies have implicated the microbiome in certain diseases, especially chronic diseases. In this study, the bacterial communities in the sputum of pulmonary tuberculosis patients were explored. Total DNA was extracted from sputum samples from 31 pulmonary tuberculosis patients and respiratory secretions of 24 healthy participants. The 16S rRNA V3 hyper-variable regions were amplified using bar-coded primers and pyro-sequenced using Roche 454 FLX. The results showed that the microbiota in the sputum of pulmonary tuberculosis patients were more diverse than those of healthy participants (p