EFFECT OF miR-429 ON THE PROLIFERATION AND MIGRATION OF BREAST CANCER CELLS AND ITS MECHANISM

Objective To investigate the effect of miR-429 on the proliferation and migration of breast cancer cells and its mechanism. Methods RT-qPCR and Western blotting were used to measure the relative mRNA and protein expression levels of eIF4E in breast cancer MCF-7, MDA-MB-231, MDA-MB-468, and BT-549 cells and normal breast epithelial MCF-10A cells. MCF-7 cells were transfected with miR-429 mimics (group A), mimics NC (group B), miR-429 inhibitors (group C), and inhibitors NC (group D), and RT-qPCR, CCK-8 assay, wound healing assay, and Western blotting were used to measure the expression of miR-429, cell proliferation and migration, and the relative mRNA and protein expression levels of eIF4E in groups A-D. The 293T cells were transfected with eIF4E-3'UTR-Wt+miR-429 mimics (group E), eIF4E-3'UTR-Wt+mimics NC (group F), eIF4E-3'UTR-Mut+miR-429 mimics (group G), and eIF4E-3'UTR-Mut+mimics NC (group H), and relative luciferase activity was measured for groups E-H. The targeted regulatory effect of miR-429 on eIF4E