Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing

We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenom...

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Veröffentlicht in:	Scientific reports 2019-09, Vol.9 (1), p.12943-10, Article 12943
Hauptverfasser:	Xue, Vivian W., Cheung, Moon T., Chan, Pak T., Luk, Lewis L. Y., Lee, Vivian H., Au, Thomas C., Yu, Allen C., Cho, William C. S., Tsang, Hin Fung Andy, Chan, Amanda K., Wong, S. C. Cesar
Format:	Artikel
Sprache:	eng
Schlagworte:	38 38/91 45 692/4028/67 692/53/2421 Adenoma Adenoma - blood Adenoma - genetics Adenoma - pathology Apoptosis Biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Case-Control Studies Cell death Cell differentiation Colon Colorectal cancer Colorectal Neoplasms - blood Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic Humanities and Social Sciences Humans mRNA multidisciplinary Prognosis RhoA protein RNA, Messenger - blood RNA, Messenger - genetics Science Science (multidisciplinary) Tumors
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Zusammenfassung:	We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-019-49445-x