Efferocytosis requires periphagosomal Ca2+-signaling and TRPM7-mediated electrical activity
Efficient clearance of apoptotic cells by phagocytosis, also known as efferocytosis, is fundamental to developmental biology, organ physiology, and immunology. Macrophages use multiple mechanisms to detect and engulf apoptotic cells, but the signaling pathways that regulate the digestion of the apop...
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Veröffentlicht in: | Nature communications 2022-06, Vol.13 (1), p.3230-3230, Article 3230 |
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Sprache: | eng |
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Zusammenfassung: | Efficient clearance of apoptotic cells by phagocytosis, also known as efferocytosis, is fundamental to developmental biology, organ physiology, and immunology. Macrophages use multiple mechanisms to detect and engulf apoptotic cells, but the signaling pathways that regulate the digestion of the apoptotic cell cargo, such as the dynamic Ca
2+
signals, are poorly understood. Using an siRNA screen, we identify TRPM7 as a Ca
2+
-conducting ion channel essential for phagosome maturation during efferocytosis.
Trpm7
-targeted macrophages fail to fully acidify or digest their phagosomal cargo in the absence of TRPM7. Through perforated patch electrophysiology, we demonstrate that TRPM7 mediates a pH-activated cationic current necessary to sustain phagosomal acidification. Using mice expressing a genetically-encoded Ca
2+
sensor, we observe that phagosome maturation requires peri-phagosomal Ca
2+
-signals dependent on TRPM7. Overall, we reveal TRPM7 as a central regulator of phagosome maturation during macrophage efferocytosis.
Efficient removal of apoptotic cells by phagocytosis underlies tissue development, wound repair, host defense and organ homeostasis. Here, authors identify TRPM7 as a regulator of cargo acidification and Ca2+ signaling during apoptotic cell clearance. |
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ISSN: | 2041-1723 2041-1723 |
DOI: | 10.1038/s41467-022-30959-4 |