FLUORO QUINOLONE INFLUENCE UPON STAPHYLOCOCCUS ЕPIDERMIDIS BIOFILM

Aim. To study the effect of ofloxacin and levofloxacin on biofilm of Staphylococcus epidermidis clinical strains. Methods. Resistance to antibiotics was determined by the method of discs with fluoroquinolones of different generations: nalidixic acid, pipemidyn acid, ciprofloxacin, ofloxacin, norfloxacin, levofloxacin, sparfloxacin. The formation of biofilm by staphylococci modeled minimum inhibitory concentration (MIC) of antibiotics for planktonic culture, it was determined by the method of serial dilutions. The formation of biofilm by staphylococci was modeled in immunological well plates. Results. It is shown that ofloxacin in concentrations 100 times higher than the MIC for biofilm formation reduces the bacteria on 24 hours biofilm more than 40000 times, on 48 hour – 31000 times, the application of the same concentration of levofloxacin to 24 hours biofilm – in 9100 times, to 48 hours biofilm – in 7400 times. Conclusions. Ofloxacin in the concentration exceeding 100 times the MIC for biofilm formation by 4.5 and 4.2 times more effective against 24 hour and 48 hour biofilm, respectively, compared with the same concentration of levofloxacin.