The effect of LNCRNA SHANK3 on the malignant development of gastric cancer cells by regulating the miR-4530/MNX1

•LNCRNA SHANK3 is highly expressed in gastric cancer and is associated with poor prognosis.•LNCRNA SHANK3 sponge adsorbs miR-4530 to inhibit its expression, while mine-4530 inhibits its expression by binding to 3 'UTR of MNX1.•LNCRNA SHANK3 regulates the proliferation, migration, and invasion of gastric cancer cells by regulating miR-4530/MNX1.•This study has the hope of providing possible diagnostic and therapeutic targets and theoretical support for the design and development of GC preventive drugs. Gastric cancer (GC) has become the first malignant tumor with highest incidence rate and mortality of cancer in China, finding therapeutic targets for gastric cancer is of great significant for improving the survival rate of patients with GC. Recently, many of studies have shown that LncRNAs is involved in multiple biological progresses in the development of GC. This study, we screened for abnormally high expression of LncSHANK3 in GC through the TCGA database, and found that LncSHANK3 sponge adsorbs miR-4530, further competing with MNX1 and binding to miR-4530. We demonstrated the interaction between LncSHANK3 and miR-4530 through luciferase reporting analysis, with miR-4530 negatively regulating MNX1.Through CCK8, colony formation, transwell, and wound healing assays, it was found that LncSHANK3 affects the occurrence of GC through cell proliferation, migration and invasion. In conclusion, LncSHANK3/miR-4530/MNX1 axis is a potential mechanism for the treatment of GC.