Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge

Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge

Section Highlights Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety.

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Veröffentlicht in:Molecular cancer 2022-06, Vol.21 (1), p.1-136, Article 136
Hauptverfasser: Jiang, Junfeng, Zeng, Tao, Zhang, Li, Fan, Xingfei, Jin, Qishu, Ni, Haitao, Ye, Yusheng, Cheng, Lipeng, Li, Li, Wang, Liujun, Xu, Sha, Yang, Yu, Gu, Juan, Guo, Bing, Wang, Lei, Li, Xin, Qin, Yingyi, Li, Jiaxi, Wang, Jinjiang, Chen, Xi, Wu, Minjuan, Ying, Qi-long, Qin, Xingjun, Wang, Yefei, Wang, Yue
Format: Artikel
Sprache:eng
Schlagworte:
Binding sites
Cancer
Cell cycle
Cell growth
CRISPR
CRISPR–Cas9
Gene expression
Hypotheses
Let-7
Letter to the Editor
MicroRNAs
miRNA
miRNA sponge
Mutation
Nucleotide sequence
Optimization
Ribonucleic acid
RNA
RNA sequence optimization
Software
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Beschreibung
Zusammenfassung:Section Highlights Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety.
ISSN:1476-4598
1476-4598
DOI:10.1186/s12943-022-01604-x