Generation of cytochrome P450 enzyme-polymorphic human induced pluripotent stem cell lines with CYP2D610/CYP3A53C and CYP2D610/CYP2C192

Cytochrome P450 (CYP) reaction phenotyping has become crucial for predicting drug reactions and side effects. Single nucleotide polymorphisms (SNPs) in CYP genes alter drug metabolism capacity and cause unexpected drug-related reactions. Here, we established two human induced pluripotent stem cell (...

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Veröffentlicht in:	Stem cell research 2022-12, Vol.65, p.102970-102970, Article 102970
Hauptverfasser:	Lee, Jaehun, Kim, Gyeongmin, Chi, Kyun Yoo, Han, Choongseong, Kim, Jong-Hoon
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line Cytochrome P-450 CYP2C19 - genetics Cytochrome P-450 CYP2D6 - genetics Cytochrome P-450 CYP3A - genetics Humans Induced Pluripotent Stem Cells - enzymology Pharmaceutical Preparations - metabolism Polymorphism, Single Nucleotide
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Zusammenfassung:	Cytochrome P450 (CYP) reaction phenotyping has become crucial for predicting drug reactions and side effects. Single nucleotide polymorphisms (SNPs) in CYP genes alter drug metabolism capacity and cause unexpected drug-related reactions. Here, we established two human induced pluripotent stem cell (hiPSC) lines with pharmacologically important SNPs in CYP2D6 in conjunction with CYP2C19 or CYP3A5 genes. These hiPSC lines can serve as valuable resources for expanding our understanding of the relationships between genotypes and drug reactions.
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2022.102970