Identification and Growth Characterization of a Novel Strain of Saccharomyces boulardii Isolated From Soya Paste

The nonpathogenic yeast (Sb) has beneficial effects on the human intestine, and thus has been prescribed as probiotics for the treatment of diarrhea and gastrointestinal diseases. This is the only commercialized yeast with the purpose of being used as human medicine. Currently, little is known about their multiple mechanisms of actions. The yeast strain is isolated and identified by using the BIOLOG microarray identification system and morphologically. To understand its functional roles, the present study investigates the ability of this yeast to tolerate different concentrations of bile salt up to 2.5%, cell hydrophobicity, antioxidants, autoaggregation activity, and simulated gastrointestinal digestion. The effect of temperatures (up to 50°C), pH (up to 8.0), and salinity (at best 7%) was also monitored on the growth and survival of the yeast cell. The physicochemical analyses revealed that could survive in stomach conditions at pH 2.5, temperature 37°C, and 2% bile salt. Antibiotic susceptibility of was carried out using commercial antibiotic discs. The antimicrobial activity of the isolated against bacterial pathogens related to diarrhea diseases was determined by the Well Diffusion method. The biosafety assay findings also claimed could be a potential probiotic. The experimental findings suggest that the isolated possesses excellent probiotic capacities as a biotherapeutic agent for antidiarrheal and gastrointestinal disorders.