Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

The pathogenic bacterium Brucella abortus codes for a multi-domain dimeric cytoplasmic histidine kinase called LOV-HK, which is a key blue light-activated virulence factor in this microorganism. The structural basis of the light activation mechanism of this protein remains unclear. In this work, ful...

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Veröffentlicht in:Biochemistry and biophysics reports 2018-12, Vol.16, p.39-43
Hauptverfasser: Rinaldi, Jimena, Fernández, Ignacio, Poth, Lucía M., Shepard, William E., Savko, Martin, Goldbaum, Fernando A., Klinke, Sebastián
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Sprache:eng
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Zusammenfassung:The pathogenic bacterium Brucella abortus codes for a multi-domain dimeric cytoplasmic histidine kinase called LOV-HK, which is a key blue light-activated virulence factor in this microorganism. The structural basis of the light activation mechanism of this protein remains unclear. In this work, full-length LOV-HK was cloned, expressed and purified. The protein was activated by light and crystallized under a controlled illumination environment. The merge of 14 individual native data sets collected on a single crystal resulted in a complete X-ray diffraction data set to a resolution of 3.70 Å with over 2 million reflections. Crystals belong to space group P212121, with unit-cell parameters a = 95.96, b = 105.30, c = 164.49 Å with a dimer in the asymmetric unit. Molecular replacement with Phaser using the individual domains as search models allowed for the reconstruction of almost the whole protein. Very recently, improved LOV-HK crystals led to a 3.25-Å resolution dataset. Refinement and model building is underway. This crystal model will represent one of the very few examples of a multi-domain histidine kinase with known structure. [Display omitted] •The LOV-HK histidine kinase from Brucella was crystallized in its activated state.•A strict illumination control was followed for protein crystallization.•Highly redundant X-ray diffraction data were collected at 3.70 Å maximum resolution.•Phasing was achieved in a tricky molecular replacement case.
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2018.09.005