Ecology of fear in highly invasive fish revealed by robots

Invasive species threaten biodiversity and ecosystem functioning. We develop an innovative experimental approach, integrating biologically inspired robotics, time-series analysis, and computer vision, to build a detailed profile of the effects of non-lethal stress on the ecology and evolution of mosquitofish (Gambusia holbrooki)—a global pest. We reveal that brief exposures to a robotic predator alter mosquitofish behavior, increasing fear and stress responses, and mitigate the impact of mosquitofish on native tadpoles (Litoria moorei) in a cause-and-effect fashion. Effects of predation risk from the robot carry over to routine activity and feeding rate of mosquitofish weeks after exposure, resulting in weight loss, variation in body shape, and reduction in the fertility of both sexes—impairing survival, reproduction, and ecological success. We capitalize on evolved responses of mosquitofish to reduce predation risk—neglected in biological control practices—and provide scientific foundations for widespread use of state-of-the-art robotics in ecology and evolution research. [Display omitted] •Can robotic predators reveal the vulnerabilities of invasive and pest species?•Our predator selectively targets invasive fish to protect native amphibians•Stress from the robot compromises behavior, health, and reproduction of invaders•We open new frontiers for robotics in ecology, evolution, and biocontrol research Biological Sciences, Ecology, zoology