Obligately aerobic human gut microbe expresses an oxygen resistant tungsten-containing oxidoreductase for detoxifying gut aldehydes

Obligately aerobic human gut microbe expresses an oxygen resistant tungsten-containing oxidoreductase for detoxifying gut aldehydes

strain phR is an obligately aerobic microbe that was isolated from human feces. Here, we show that it readily takes up tungsten (W), a metal previously associated only with anaerobes. The W is incorporated into an oxidoreductase enzyme (BmWOR) that was purified from native biomass. BmWOR consists of...

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Veröffentlicht in:Frontiers in microbiology 2022-08, Vol.13, p.965625-965625
Hauptverfasser: Thorgersen, Michael P, Schut, Gerrit J, Poole, 2nd, Farris L, Haja, Dominik K, Putumbaka, Saisuki, Mycroft, Harriet I, de Vries, Willem J, Adams, Michael W W
Format: Artikel
Sprache:eng
Schlagworte:
aerobe
aldehyde oxidation
benzaldehyde
human gut microbiome
Microbiology
tungsten
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Zusammenfassung:strain phR is an obligately aerobic microbe that was isolated from human feces. Here, we show that it readily takes up tungsten (W), a metal previously associated only with anaerobes. The W is incorporated into an oxidoreductase enzyme (BmWOR) that was purified from native biomass. BmWOR consists of a single 65 kDa subunit and contains a single W-pyranopterin cofactor and a single [4Fe-4S] cluster. It exhibited high aldehyde-oxidizing activity with very high affinities (apparent K  
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.965625