AHR inhibitor CH223191 improves the effect of bone marrow transplantation in irradiated mice

Objective To investigate the effect of radiation-induced bystander effect on donor bone marrow cells during bone marrow transplantation and the protective effect of aryl hydrocarbon receptor (AHR) inhibitor CH223191 on donor bone marrow cells. Methods The mice were divided into 9 Gy irradiated group and 12 Gy irradiated group. The same number of donor bone marrow cells were transplanted after different radiation doses. The total number of peripheral blood cells, chimerism rate and number of peripheral blood donor cells were detected by flow cytometry. Serum reactive oxygen species (ROS) level of unirradiated mice and irradiated mice were detected by ELISA. The donor bone marrow cells were divided into control group, 9 Gy irradiated group and 12 Gy irradiated group and were cultured in vitro. Unirradiated mouse serum, 9 Gy and 12 Gy irradiated mouse serum were added for treatment, respectively. ATP activity assay was used to detect cell vitality, and flow cytometry was used to detect cell apoptosis. The mRNA e