Annexin- and calcium-regulated priming of legume root cells for endosymbiotic infection

Legumes establish endosymbioses with arbuscular mycorrhizal (AM) fungi or rhizobia bacteria to improve mineral nutrition. Symbionts are hosted in privileged habitats, root cortex (for AM fungi) or nodules (for rhizobia) for efficient nutrient exchange. To reach these habitats, plants form cytoplasmi...

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Veröffentlicht in:Nature communications 2024-12, Vol.15 (1), p.10639-21, Article 10639
Hauptverfasser: Guillory, Ambre, Fournier, Joëlle, Kelner, Audrey, Hobecker, Karen, Auriac, Marie-Christine, Frances, Lisa, Delers, Anaïs, Pedinotti, Léa, Le Ru, Aurélie, Keller, Jean, Delaux, Pierre-Marc, Gutjahr, Caroline, Frei Dit Frey, Nicolas, de Carvalho-Niebel, Fernanda
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Sprache:eng
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Zusammenfassung:Legumes establish endosymbioses with arbuscular mycorrhizal (AM) fungi or rhizobia bacteria to improve mineral nutrition. Symbionts are hosted in privileged habitats, root cortex (for AM fungi) or nodules (for rhizobia) for efficient nutrient exchange. To reach these habitats, plants form cytoplasmic cell bridges, key to predicting and guiding fungal hyphae or rhizobia-filled infection thread (IT) root entry. However, the underlying mechanisms are poorly studied. Here we show that unique ultrastructural changes and calcium (Ca 2+ ) spiking signatures, closely associated with Medicago truncatula Annexin 1 (MtAnn1) accumulation, accompany rhizobia-related bridge formation. Loss of MtAnn1 function in M. truncatula affects Ca 2+ spike amplitude, cytoplasmic configuration and rhizobia infection efficiency, consistent with a role of MtAnn1 in regulating infection priming. MtAnn1 , which evolved in species establishing intracellular symbioses, is also AM-symbiosis-induced and required for proper arbuscule formation. Together, we propose that MtAnn1 is part of an ancient Ca 2+ -regulatory module for transcellular endosymbiotic infection. This study describes ultrastructural changes and cell-specific calcium responses that accompany cytoplasmic bridge formation in legume root cells committed to symbiotic rhizobial infection, with fine-tuning by a calcium-binding annexin.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-024-55067-3