Immunofluorescence assay for serologic diagnosis of SARS

We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no contr...

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Veröffentlicht in:	Emerging infectious diseases 2004-03, Vol.10 (3), p.530-532
Hauptverfasser:	Chan, Paul K S, Ng, King-Cheung, Chan, Rickjason C W, Lam, Rebecca K Y, Chow, Viola C Y, Hui, Mamie, Wu, Alan, Lee, Nelson, Yap, Florence H Y, Cheng, Frankie W T, Sung, Joseph J Y, Tam, John S
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Antibodies, Viral - isolation & purification Child Child, Preschool Communicable Diseases, Emerging - diagnosis Communicable Diseases, Emerging - immunology Communicable Diseases, Emerging - physiopathology coronavirus diagnosis Dispatch Female Fluorescent Antibody Technique, Indirect - methods Hong Kong Humans Male Middle Aged Reverse Transcriptase Polymerase Chain Reaction sensitivity serology Severe acute respiratory syndrome Severe Acute Respiratory Syndrome - diagnosis Severe Acute Respiratory Syndrome - immunology Severe Acute Respiratory Syndrome - physiopathology specificity
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Zusammenfassung:	We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid1003.030493