LRCH1 maintains the function of regulatory T cells in mouse models of lupus nephritis

Objective To evaluate the expression and effect of leucine rich repeats and calponin homology domain containing 1 (LRCH1) in regulatory T cells (Tregs) from lupus nephritis. Methods The 6-month old C57BL/6 and MRL/lpr mice were used to enrich CD45+CD4+CD25+CD127low Tregs from the spleens and kidneys by flow cytometry. RT-qPCR was applied to measure LRCH1 mRNA in these cells. Exogenous Tregs were adoptively transferred into C57BL/6 mice and MRL/lpr mice, followed by detection of mRNA of LRCH1,IL-10. TGFβ(transforming growth factor beta) in exogenous Tregs in the spleen and kidney of recipient mice using RT-qPCR. Furthermore, normal Tregs were transfected with siRNAs to silence Lrch1 expression and followed by the evaluation of Treg-induced suppression of conventional T cell proliferation with flow cytometry. Immunoblotting was performed to find the activation of IL-2 signaling. Results Compared with wild type mice, Tregs up-regulated LRCH1 by 4.13 folds in the kidney of MRL/lpr mouse models. The adoptive trans