Effects of benzo[a]pyrene on gilthead seabream (Sparus aurata L.) hepatocytes exposed in vitro to short and long term trials

In the present study, cytotoxic effects to the polycyclic aromatic hydrocarbons benzo[a]pyrene (B[a]P) were investigated in Sparus aurata hepatocytes primary cultures after acute and chronic exposure. Cells were treated with a wide range of B[a]P doses (100 µg/ml to 1 pg/ml) for 24, 48 and 72 h. B[a...

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Veröffentlicht in:Italian journal of animal science 2013-03, Vol.12 (1), p.e17-e17
Hauptverfasser: Zacchino, Valentina, Centoducati, Gerardo, Narracci, Marcella, Selvaggi, Maria, Santacroce, Maria Pia
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Sprache:eng
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Zusammenfassung:In the present study, cytotoxic effects to the polycyclic aromatic hydrocarbons benzo[a]pyrene (B[a]P) were investigated in Sparus aurata hepatocytes primary cultures after acute and chronic exposure. Cells were treated with a wide range of B[a]P doses (100 µg/ml to 1 pg/ml) for 24, 48 and 72 h. B[a]P toxicity was quantified in seabream hepatocytes by MTT assay and immunofluorescence analysis after the various exposure periods, in order to evaluate the hepatic damage and toxicity range. Results showed three cytotoxic responses: B[a]P cell death for primary necrosis after exposure to high concentrations for short times, apoptosis induction with the use of sublethal doses and cell proliferation allied with tumoral foci formation after exposure to low concentrations for long times. This responses provided an interesting correlation between the damage caused on hepatocytes and the metabolism of this toxic, mainly studied in vivo. Addictionally, the statistical analysis revealed that the effects of time and dose were significant for both parameters and especially the time was extremely significant (p
ISSN:1594-4077
1828-051X
DOI:10.4081/ijas.2013.e17