Molecular xenomonitoring of Wuchereria bancrofti in Culex quinquefasciatus from an endemic area: Comparison of two DNA extraction methods for realtime PCR assay

In this study, we have demonstrated for the first time the performance of LDR real-time PCR assay in amplifying the genomic DNA of W. bancrofti extracted by a simple TE based method in comparison with Qiagen (standard) method for detecting filarial infection in large number of field collected mosquito pools from an endemic area in a cost-effective manner. Cost comparison in our laboratory showed that the TE based extraction of genomic DNA saves the cost (12 times) and the time (75%)8. [...]TE based extraction of DNA could be used as an alternative method as it could be less expensive and time saving while processing large number of mosquito samples by real-time PCR during post-MDA surveillance and monitoring.