Myosin VII, USH1C, and ANKS4B or USH1G Together Form Condensed Molecular Assembly via Liquid-Liquid Phase Separation

Hair cell stereocilia tip-links function to sense mechanical forces generated by sound waves and maintain the structure of stereocilia by rooting the tail of cadherins to highly dense structures known as tip-link densities. Although the molecular components are largely known, the mechanisms underlying the tip-link density formation are unknown. Here, we show that Myosin VIIB (MYO7B), USH1C, and ANKS4B, which form a specific complex stabilizing tip-links in intestine microvilli, could form dense condensates via liquid-liquid phase separation in vitro and in cells. The MYO7A, USH1C, and USH1G complex also undergoes phase separation in cells. Formation of the MYO7A/USH1C/USH1G and MYO7B/USH1C/ANKS4B condensates requires strong and multivalent interactions between proteins in both tripartite complexes. Point mutations of MYO7A found in Usher syndrome patients weaken or even disrupt the multivalent interactions of the MYO7A/USH1C/USH1G complex and impair its phase separation. Thus, the stereocilia tip-link densities may form via phase separation of the MYO7A/USH1C/USH1G complex. [Display omitted] •Microvilli tip-link proteins MYO7B, USH1C, and ANKS4B condensate via phase separation•Hair cell tip-link proteins MYO7A, USH1C, and USH1G also form condensates•Strong multivalent interactions are required for tip-link condensate formation•MYO7A mutations in USH1 patients impair MYO7A/USH1C/USH1G condensate formation He et al. report that the MYO7A/USH1C/USH1G and MYO7B/USH1C/ANKS4B complexes in hair cell stereocilia and in enterocyte microvilli form high-density protein condensates via liquid-liquid phase separation, likely for the stable rooting of tip-link structures.