c-kit+ cells offer hopes in ameliorating asthmatic pathologies via regulation of miRNA-133 and -126

Objective(s): There are still challenges regarding c-kit+ cells’ therapeutic outcome in the clinical setting. Here, we examined the c-kit+ cell effect on the alleviation of asthma by modulating miRNAs expression. Materials and Methods: To induce asthma, male rats were exposed to ovalbumin. Bone marr...

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Veröffentlicht in:Iranian journal of basic medical sciences 2021-03, Vol.24 (3), p.369-376
Hauptverfasser: Rahbarghazi, Reza, Rana Kihanmanesh, Rezaie, Jafar, Mirershadi, Fatemeh, Hossain Heiran, Hesam Saghaei Bagheri, Saberianpour, Shirin, Bagheri, Yasin, Rajabi, Hadi
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Sprache:eng
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Zusammenfassung:Objective(s): There are still challenges regarding c-kit+ cells’ therapeutic outcome in the clinical setting. Here, we examined the c-kit+ cell effect on the alleviation of asthma by modulating miRNAs expression. Materials and Methods: To induce asthma, male rats were exposed to ovalbumin. Bone marrow-derived c-kit+ cells were enriched by MACS. Animals were classified into four groups (6 rats each). Control rats received PBS intratracheally; Ovalbumin-sensitized rats received PBS intratracheally; Ovalbumin-sensitized rats received PBS intratracheally containing 3×105 c-kit+ and c-kit- cells. Cells were stained with Dil fluorescent dye to track in vivo condition. Pathological changes were monitored in asthmatic rats after transplantation of c-kit+ and c-kit- cells. Serum levels of IL-4 and INF-γ were measured by ELISA. Transcription of miRNAs (-126 and 133) was assessed by real-time PCR analysis. Results: Pathological examination and Th1 and Th2 associated cytokine fluctuation confirmed the occurrence of asthma in rats indicated by chronic changes and prominent inflammation compared with the control group (p
ISSN:2008-3866
2008-3874
DOI:10.22038/ijbms.2021.49008.11231