KARAKTERISASI ISOLAT BAKTERI ASAM LAKTAT DARI MANDAI YANG BERPOTENSI SEBAGAI PROBIOTIK (Characterization of Lactic Acid Bacteria Isolates from Mandai Function as Probiotic)

is a fermented product made of cempedak () dami. The research aimed to isolate and characterize lactic acid bacteria isolated from and to evaluate their probiotic potency. samples were collected from several home industries in East Kalimantan area lactic acid bacteria (LAB) isolates were obtained from on day 4, 8, and 12 fermentation and assessed for their probiotic properties. All isolates showed good tolerances towards low pH (pH 2.0) with the decrease of viable counts of less than 2 log cfu/ml. The LAB isolates could grow in the present of 0.5% bile salt although the viable counts decreased as compared to those in medium without bile salt. Reduction of viable counts less than 1 log cfu/ml was observed in 21 isolates. Nineteen isolates could tolerate pH 2.0 and 0.5% bile salt better than others with the totaldecrease in viable counts less than 1 log cfu/ml. Most of isolates (11 out of 19 isolates) which tolerate low pH were obtained from 8 days fermentation. Isolate MC812 and MC809 had good antimicrobial properties against ATCC 13932, ATCC 19433, ATCC 10876, ATCC 25922 and ATCC 14028. Ten isolates had good antimicrobial properties againstis a fermented product made of cempedak () dami. The research aimed to isolate and characterize lactic acid bacteria isolated from and to evaluate their probiotic potency. samples were collected from several home industries in East Kalimantan area lactic acid bacteria (LAB) isolates were obtained from on day 4, 8, and 12 fermentation and assessed for their probiotic properties. All isolates showed good tolerances towards low pH (pH 2.0) with the decrease of viable counts of less than 2 log cfu/ml. The LAB isolates could grow in the present of 0.5% bile salt although the viable counts decreased as compared to those in medium without bile salt. Reduction of viable counts less than 1 log cfu/ml was observed in 21 isolates. Nineteen isolates could tolerate pH 2.0 and 0.5% bile salt better than others with the totaldecrease in viable counts less than 1 log cfu/ml. Most of isolates (11 out of 19 isolates) which tolerate low pH were obtained from 8 days fermentation. Isolate MC812 and MC809 had good antimicrobial properties against ATCC 13932, ATCC 19433, ATCC 10876, ATCC 25922 and ATCC 14028. Ten isolates had good antimicrobial properties against LAB isolates obtained from show promising probiotic properties.Keywords: Probiotic, lactic acid bacteria, antimicrobial ABSTRAKMandai merupakan pangan ferm