The protective effect of IL‐12/23 neutralizing antibody in sarcopenia associated with dextran sulfate sodium‐induced experimental colitis

Background The improvement of colitis symptoms by treatment with IL‐12/23 p40 neutralizing antibody should increase the muscle mass and the function of the sarcopenia phenotype. Methods An experimental colitis model was induced by oral administration of 2% dextran sulfate sodium (DSS) for 7 days. During induction of colitis, IL‐12/23 p40 neutralizing antibody was injected twice on Days 3 and 5. The total body mass index was measured by dual‐energy X‐ray absorptiometry. The muscle function was measured by forelimb grip strength and fatigue running distance. The muscle fibre cross‐sectional area (CSA) was calculated after the transverse section and haematoxylin and eosin staining, and gene expression was confirmed by RT‐qPCR. Differentiated C2C12 cells were used as in vitro models and treated with recombinant IL12/23 proteins to mimic the enhanced cytokines in colitis. Results The symptoms of colitis were alleviated by injection of IL‐12/23 p40 neutralizing antibody compared with phosphate‐buffered saline (PBS), and the disease activity index score was significantly lower on Day 8 (0.0 ± 0.00 of cont. vs. 11.3 ± 0.9 of DSS + PBS, P