Unveiling directional physiological coupling in human-horse interactions

This research investigates the human-horse bond, aiming to unveil the physiological mechanisms regulating interspecies interactions. We hypothesized observing a physiological synchronization in human-horse dynamics, akin to human interactions. Through time-frequency Granger causality analysis of heart rate variability (HRV) and behavioral data, this study reveals the establishment of bidirectional synchronization in HRV between humans and horses. The coupling directionality is influenced by behavior and familiarity. In exploration scenarios led by horses, bidirectional interactions occur, particularly with familiar individuals. Conversely, during human-led activities such as grooming, physiological connectivity direction varies based on the familiarity level. In addition, the methodology allows in-depth analysis of sympathetic and parasympathetic nervous system contributions, highlighting their intricate role in the human-horse relationship. Such a physiological coupling estimate, correlated with behavioral data, provides a quantitative tool applicable across contexts and species This holds significant promise for assessing animal-assisted therapies and for applications in sports and various animal-related domains. [Display omitted] •Demonstrated bidirectional HRV synchronization between humans and horses•Causality of physiological coupling varies by familiarity and interaction type•Physiological synchronization is linked to specific behavioral dynamics•Broad applications in animal-assisted therapy and human-animal interaction studies Equine behavior; Zoology; Behavioral neuroscience