What happens when the lights are left on? Transcriptomic and phenotypic habituation to light pollution

Artificial light at night (ALAN) is a ubiquitous pollutant worldwide. Exposure can induce immediate behavioral and physiological changes in animals, sometimes leading to severe health consequences. Nevertheless, many organisms persist in light-polluted environments and may have mechanisms of habituating, reducing responses to repeated exposure over time, but this has yet to be tested experimentally. Here, we tested whether zebra finches (Taeniopygia guttata) can habituate to dim (0.3 lux) ALAN, measuring behavior, physiology (oxidative stress and telomere attrition), and gene expression in a repeated measures design, over 6 months. We present evidence of tolerance to chronic exposure, persistent behavioral responses lasting 8 weeks post-exposure, and attenuation of responses to re-exposure. Oxidative stress decreased under chronic ALAN. Changes in the blood transcriptome revealed unique responses to past exposure and re-exposure. Results demonstrate organismal resilience to chronic stressors and shed light on the capacity of birds to persist in an increasingly light-polluted world. [Display omitted] •Zebra finches show behavioral habituation to repeated light pollution exposure•Slight decrease in oxidative stress detected after chronic (8 weeks) ALAN exposure•No detected differences among treatment groups in telomere attrition•Blood transcriptome reveals unique responses to naive, past, and repeated exposure Genetics; Evolutionary biology; Animal Physiology