Development and validation of analytical method for the estimation of lamivudine in rabbit plasma

Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography （RP-HPLC） method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column （250 mm ＊ 4.6 mm, 5 μm） using a mobile phase of 0.25% Triethylamine buffer （pH 3.0）： acetonitrile （70：30, v/v）. The efficient was monitored by UV detector at 256 nm. The total run time was 15 min with a flow rate of 1.0 mL/min. Calibration curve was linear over the concentration range of 25-2000 ng/mL. The retention times of lamivudine and internal standard （Nelfinavir） were 8.78 min and 10.86 min, respectively. The developed RP-HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model.