Probiotics formulation and cancer nanovaccines show synergistic effect in immunotherapy and prevention of colon cancer

Probiotics play essential roles in immune modulation. Combining probiotics with cancer vaccines potentially can achieve a synergistic effect. To maximize the efficacy of probiotics, proper probiotics formulation is necessary. Herein, Lactobacillus rhamnosus and Bifidobacterium longum are coated with lipid membrane to achieve the goal of losing less activity and bettering colonization in colon. In the subcutaneous transplanted colon cancer mouse model, probiotics formulation showed potent preventive and therapeutic efficacy, and the efficacy could be further improved by combining with cancer nanovaccines. Probiotics formulation can perform as immune adjuvants to enhance the innate immune response or as in-situ cancer vaccines. In the study of preventing chemical-induced orthotopic colon cancer model, probiotics formulation alone efficiently reduced tumor number in colon and the efficacy is improved by combining with cancer nanovaccines. All in all, the studies demonstrated that probiotics formulation can assist to maximize the efficacy of cancer nanovaccines. [Display omitted] •Probiotics coated with lipid membrane can facilitate the cancer therapy and prevention•Proper probiotics formulation combine with cancer vaccine can prevent and treat cancer•The probiotics facilitated the induction of tumor antigen-specific cytotoxic T cells•Probiotics formulation can alter the microenvironment in colon Nanocomplex; Biological sciences; Immunology; Microbiology; Biotechnology; Cancer