Evaluation of Pyrophosphate-Driven Proton Pumps in Saccharomyces cerevisiae under Stress Conditions
In , pH homeostasis is reliant on ATP due to the use of proton-translocating ATPase (H -ATPase) which constitutes a major drain within cellular ATP supply. Here, an exogenous proton-translocating pyrophosphatase (H -PPase) from which uses inorganic pyrophosphate (PP ) rather than ATP, was evaluated...
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Veröffentlicht in: | Microorganisms (Basel) 2024-03, Vol.12 (3), p.625 |
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Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In
, pH homeostasis is reliant on ATP due to the use of proton-translocating ATPase (H
-ATPase) which constitutes a major drain within cellular ATP supply. Here, an exogenous proton-translocating pyrophosphatase (H
-PPase) from
which uses inorganic pyrophosphate (PP
) rather than ATP, was evaluated for its effect on reducing the ATP burden. The H
-Ppase was localized to the vacuolar membrane or to the cell membrane, and their impact was studied under acetate stress at a low pH. Biosensors (pHluorin and mQueen-2m) were used to observe changes in intracellular pH (pH
) and ATP levels during growth on either glucose or xylose. A significant improvement of 35% in the growth rate at a pH of 3.7 and 6 g·L
acetic acid stress was observed in the vacuolar membrane H
-PPase strain compared to the parent strain. ATP levels were elevated in the same strain during anaerobic glucose and xylose fermentations. During anaerobic xylose fermentations, co-expression of pHluorin and a vacuolar membrane H
-PPase improved the growth characteristics by means of an improved growth rate (11.4%) and elongated logarithmic growth duration. Our study identified a potential method for improving productivity in the use of
as a cell factory under the harsh conditions present in industry. |
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ISSN: | 2076-2607 2076-2607 |
DOI: | 10.3390/microorganisms12030625 |