ANTIBIOFILM-FORMING AND ANTIMICROBIAL ACTIVITY OF EXTRACTS OF Arnica montana L., Achillea millefolium L. ON Staphylococcus GENUS BACTERIA

The purpose of this work was to study the antimicrobial, antibiofilm-forming and antioxidant properties of alcohol extracts of Arnica montana L. and Achillea millefolium L. The plants for the analysis were gathered in the territory of Velyky Berezny region, Transcarpathia. Ethyl and methyl extracts were produced from inflorescences of Arnica montana L. and Achillea millefolium L. For the purpose of analysis, we used Staphylococcus genus bacteria that had been isolated from the mouth cavities and pharynx of human patients suffering from inflammatory diseases, by plating into differentially diagnostic nutrient media with subsequent identification. All isolates were characterized to be biofilm-forming. The subject of the study was extract s antimicrobial activity evaluated by the diffusion-into-agar method in standard 96-well plates using the spectrophotometric method and by measuring of antioxidant activity (DPPH test). It was established that Arnica montana L. extracts exerted more pronounced antimicrobial activity upon the analysed isolates of Staphylococcus genus bacteria. It was furthermore shown that Arnica montana L. extracts displayed an antimicrobial effect even upon MRSA of S. aureus. Extracts of Arnica montana L. and Achillea millefolium L. were shown to possess anti-biofilm forming properties. Ethyl and methyl extracts of Arnica montana L. and Achillea millefolium L. were shown to reveal significant antioxidant activity. Thus, our results indicated a need in further research of possible application of Arnica montana L. and Achillea millefolium L. extracts as anti-staphylococcal agents, which could be employed for the treatment of inflammatory processes in mouth cavity and oropharynx.