Comparison and optimization of DNA Isolation protocols for high throughput genomic studies of Acacia pachyceras Schwartz

Comparison and optimization of DNA Isolation protocols for high throughput genomic studies of Acacia pachyceras Schwartz

We describe the optimization and validation of six DNA isolation protocols from fresh leaves of the rare tree Acacia pachyceras. The first four protocols employed three commercial kits (Sigma, Nucleospin1, Nucleospin 2, Promega) whereas the remaining two were based on the traditional sodium dodecyl...

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Veröffentlicht in:MethodsX 2022-01, Vol.9, p.101799-101799, Article 101799
Hauptverfasser: Habibi, Nazima, Al Salameen, Fadila, Rahman, Muhammed, Shajan, Anisha, Zakir, Farhana, Abdulrazzack, Nasreem
Format: Artikel
Sprache:eng
Schlagworte:
DNA
Next-generation sequencing
Protocol
Tree species
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Zusammenfassung:We describe the optimization and validation of six DNA isolation protocols from fresh leaves of the rare tree Acacia pachyceras. The first four protocols employed three commercial kits (Sigma, Nucleospin1, Nucleospin 2, Promega) whereas the remaining two were based on the traditional sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide CTAB methods. Each protocol provided significantly different results concerning DNA concentration (p 
ISSN:2215-0161
2215-0161
DOI:10.1016/j.mex.2022.101799