LncRNA-ATB inhibits human keloid fibroblast proliferation and collagen synthesis

Objective To investigate the effects of lncRNA-ATB on the proliferation and collagen synthesis of human keloid fibroblast (KFB) and its possible molecular mechanism. Methods Human KFB and normal fibroblasts (NFB) were isolated and cultured in vitro, KFB was divided into NC group, si-NC group, si-lncRNA-ATB group, si-lncRNA-ATB+TNF-α group. RT-qPCR was used to detect the expression of lncRNA-ATB in NFB and KFB. MTT was used to detect cell activity. Flow cytometry was used to detect cell cycle. Western blot was used to detect expression of cyclinD1, collagen synthesis and NF-κB signaling pathway related protein. Results The expression of lncRNA-ATB in KFB cells was significantly increased (P＜0.05). Knock-down lncRNA-ATB expression decreased the A value of KFB cells, the expression of cyclinD1 and the percentageof S phase cells,but increased the percent- age of G0/G1 phase cells (P＜0.05); the expression of Col-Ⅰ and Col-Ⅲ of collagen was decreased(P＜0.05); the expression of p-p65 and p-IκBα in NF-κB signaling pa