Rhinosectan ® spray (containing xyloglucan) on the ciliary function of the nasal respiratory epithelium; results of an in vitro study

To assess the effects of Rhinosectan spray, a medical device containing xyloglucan, on nasal ciliary function (in MucilAir™Nasal cells). MucilAir™Nasal, a three-dimensional organotypic airway tissue model (with different cell types), was treated with Rhinosectan (30 µl) or with a control (saline solution). The effects of Rhinosectan were evaluated at 15 and 60 min post-exposure by: measurement of the cilia beating frequency (Hz), mucin detection (Enzyme-Linked Lectin Assay-ELLA), mucociliary clearance (µm/s) and phagocytosis assay (fluorescence). Exposure of MucilAir™ to Rhinosectan did not alter the cilia beating frequency at 15 and 60 min post-exposure (diluted and undiluted). Exposure to Rhinosectan (undiluted) during 60 min increased mucociliary clearance (93.3 ± 2.1 µm/s vs. 80.9 ± 1.8 µm/s; p