Jagged-1 inhibits mouse embryonic stem cells differentiating into haematopoietic stem or progenitor cells

Objective To investigate the effect of Notch signaling pathways on differentiation of mouse embryonic stem cells(ESC) into haematopoietic stem or progenitor cells(HSC/HPC). Methods 1)incubate mouse embryoid body cells and divide the embryonic stem cells into five groups as EB group,control group,Jagged-1 group, DAPT group and Jagged-1-DAPT group. 2)detect the phenotype of HSC/HPC by flow cytometry. 3)The expression of Notch signaling pathway, mouse embryonic stem cell phenotype and HSC/HPC phenotype were detected by RT-PCR. Results 1)the number of embryonic stem cells in Jagged-1 group was significantly more than that in control group and Jagged-DAPT group(P＜0.05);2)the number of HSC/HPC in Jagged-1-DAPT group was significantly more than that in control group and in Jagged-1 group(P＜0.05); 3)the expression of Notch1, Notch2 and Notch4 mRNA in Jagged-1 group was significantly higher than that in control group(P＜0.05), 4)the expression of Notch1 and Notch4 mRNA in DAPT group and Jagged-1-DAPT group was lower th