Long noncoding RNA Ftx regulates the protein expression profile in HCT116 human colon cancer cells

The long noncoding RNA (lncRNA) five prime to Xist (Ftx) is involved in distant metastasis in colorectal cancer (CRC). This study aimed to investigate Ftx alteration-induced proteomic changes in the highly metastatic CRC cell line HCT116. Tandem mass tag (TMT)-based proteomics analysis was performed...

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Veröffentlicht in:	Proteome science 2022-04, Vol.20 (1), p.7-7, Article 7
Hauptverfasser:	Jia, Ruzhen, Song, Lulu, Fei, Zhiqiang, Qin, Chengyong, Zhao, Qi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antisense RNA Bioinformatics Cancer Colon cancer Colorectal cancer Colorectal carcinoma Development and progression Ethylenediaminetetraacetic acid Gastric cancer Genes Genomes Genomics Long noncoding RNA Mass spectrometry Metastases Metastasis MicroRNAs Oncology, Experimental Peptides Protein expression Proteins Proteome Proteomics Scientific imaging Signal transduction Statistical analysis Therapeutic targets Tumorigenesis
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Zusammenfassung:	The long noncoding RNA (lncRNA) five prime to Xist (Ftx) is involved in distant metastasis in colorectal cancer (CRC). This study aimed to investigate Ftx alteration-induced proteomic changes in the highly metastatic CRC cell line HCT116. Tandem mass tag (TMT)-based proteomics analysis was performed to detect the differential protein expression in Ftx-overexpressing and Ftx-silenced HCT116 cells. The differentially expressed proteins were classified and characterized by bioinformatics analyses, including gene ontology (GO) annotation, GO/Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway/protein domain enrichment analyses, as well as hierarchical clustering. A total of 5471 proteins were quantified, and the proteins with \|fold change\|≥ 1.2 and p
ISSN:	1477-5956 1477-5956
DOI:	10.1186/s12953-022-00187-1