Biomphalaria alexandrina snails as immunogens against Schistosoma mansoni infection in mice
Despite effective chemotherapy, schistosomiasis remains the second largest public health problem in the developing world. Currently, vaccination is the new strategy for schistosomiasis control. The presence of common antigenic fractions between Schistosoma mansoni and its intermediate host provides...
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Veröffentlicht in: | Memórias do Instituto Oswaldo Cruz 2010-11, Vol.105 (7), p.879-888 |
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Sprache: | eng |
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Zusammenfassung: | Despite effective chemotherapy, schistosomiasis remains the second
largest public health problem in the developing world. Currently,
vaccination is the new strategy for schistosomiasis control. The
presence of common antigenic fractions between Schistosoma mansoni and
its intermediate host provides a source for the preparation of a proper
vaccine. The objective of this paper is to evaluate the nucleoprotein
extracted from either susceptible or resistant snails to protect
against schistosomiasis. The vaccination schedule consisted of a
subcutaneous injection of 50 µg protein of each antigen followed
by another inoculation 15 days later. Analyses of marker enzymes for
different cell organelles [succinate dehydrogenase, lactate
dehydrogenase (LDH), glucose-6-phosphatase, acid phosphatase and
5'-nucleotidase] were carried out. Energetic parameters (ATP, ADP, AMP,
phosphate potentials, inorganic phosphate, amino acids and LDH
isoenzymes) were also investigated. The work was extended to record
worm and ova counts, oogram determination in the liver and intestine
and the histopathological pattern of the liver. The nucleoprotein of
susceptible snails showed reduction in worm and ova counts by 70.96%
and 51.31%, respectively, whereas the nucleoprotein of resistant snails
showed |
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ISSN: | 1678-8060 0074-0276 1678-8060 0074-0276 |
DOI: | 10.1590/S0074-02762010000700007 |