Identification of Novel Bile Salt-Tolerant Genes in Lactobacillus Using Comparative Genomics and Its Application in the Rapid Screening of Tolerant Strains

Under bile salt treatment, strains display significant differences in their tolerance ability, suggesting the existence of diverse resistance mechanisms in ; however, the genes involved in this protective process are not fully understood. In this study, novel target genes associated with bile salt tolerance in were identified using comparative genomics for PCR detection and the rapid screening of tolerant strains. The bile salt tolerance of 107 isolated from different origins was assessed, and 26 strains with comparatively large differences were selected for further comparative genomic analysis. Tolerant strains had 112 specific genes that were enriched in the phosphotransferase system, the two-component system, carbohydrate metabolism, and the ATP-binding cassette transporter. Six genes from were cloned into the inducible lactobacillal expression vector pSIP403. Overexpression in the host strain increased its tolerance ability by 11.86-18.08%. The novel genes identified here can be used as targets to design primers for the rapid screening of bile salt-tolerant . Altogether, these results deepen our understanding of bile salt tolerance mechanisms in and provide a basis for further rapid assessments of tolerant strains.