Identification of novel single nucleotide polymorphisms in the PRKAG3 gene of Pakistani river buffalo

Pakistani river buffalo is classified in five breeds out of which Kundi buffalo has been least documented. This study is designed to find single nucleotide polymorphisms (SNPs) in exonic and in-tronic regions of PRKAG3 (AMP-activated, Gamma3 non-catalytic subunit) gene in the Kundi buf-falo. The PRKAG3 gene of 95 animals each from Kundi and Nili-Ravi were sequenced for identifica-tion of novel SNPs. Comparing with the Nili Ravi breed of buffalo PRKAG3 gene, six SNP sites were identified in the Kundi buffalo. The novel SNPs found in this work can function as a genomic indicator for genetic-phenotypic relationship of PRKAG3 gene with milk and meat production in buffalo. This is the first report of SNPs in PRKAG3 gene of Kundi Buffalo.